| 產品名稱 |
HFBCA08 |
| 商品貨號 |
B242206 |
| Designations |
HFBCA08 |
| GenBank Number |
M78324
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 5.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene symbol: D0S1312E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens synaptotagmin 1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: M78324; EST: EST00472; Primer: M13 Forward
DNA Seq. Acc.: T04843; EST: EST02730; Primer: M13 Reverse;
THC id: THC91295
DNA Seq. Acc.: unsubmitted; EST: EST07497; THC id: THC91295 Insert lengths(kb): 2.5 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: synaptotagmin 1(synaptotagmin (p65)) [SYT] |
| Insert Size (kb) |
2.5 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4, 1.1; PstI--3.2, 1.5, 0.35 (doublet), 0.1; HindIII--4.55, 0.95. The insert contains multiple EcoRI fragments (kb): 1.5, 1.1. It is important to note these may have been derived from different cDNAs. EST00472 matches Swiss-Prot accession SY65$HUMAN with 53.57% identity and 82.14% similarity over the sequence range: 229-312. This sequence record is for human synaptotagmin (p65) (SYT). |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434
J C Venter, personal communication
|
