Restriction digests of the clone give the following sizes (kb): EcoRI--2.2, 1.9, 0.4; XhoI--4.8; EcoRI/XhoI--2.1, 1.1, 0.98; PstI--3.3, 1.1; BglI--3.2, 1.2; EcoRI/HindIII--2.3, 1.9; NcoI--2.5, 1.9. Insert encodes fibrinogen A alpha amino acids 1-625 with an initiating ATG in front of amino acid 1. The vector is an expression vector. The depositor notes that the fibrinogen clones (ATCC 59706-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells. The clones do not encode the signal peptide necessary for assembly and secretion and one is known to lack a single nucleotide in the coding region. The ability of this probe to detect the TaqI polymorphism was verified by J. Murray, University of Iowa. |
