(1984), the B95-8 cells used to produce this strain of EBV are infected with and may also shed an uncharacterized type D retrovirus.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 5 x 1 mL vials
Storage Conditions
-70°C or colder
Comments
A 1 mL aliquot of this EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™). VR-1492 is the designation for the ATCC® set of five 1 mL vials of VR-1491. VR-1491 is EBV virus shed in the supernatant of persistently infected and transformed B95-8 cells. The EBV virus itself is of human origin. The B95-8 cells used to produce this strain of EBV are infected with and may also shed an uncharacterized type D retrovirus.
Quality control testing is done by transformation assay instead of TCID [50]
Effect on Host
EBV transforms/immortalizes human and other primate B-lymphocytes. Indicators of transformed cells include cone-shaped cells, cells with spike-like projections, and small clusters of healthy cells.
Recommended Host
B95-8 cells
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Incubate in a humidified 5% CO2 atmosphere until indicators of successful transformation appear.
Incubation: 10-25 days
Name of Depositor
G Miller
Special Collection
NCRR Contract
Source
Human lymphoblastoid cell line (883L) derived from a patient with transfusion-induced infectious mononucleosis.
References
Caputo JL, et al. An effective method for establishing Human B lymphoblastic cell lines using Epstein-Barr Virus. J. Tissue Culture Methods 13: 39-44, 1991.
Miller G, et al. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc. Natl. Acad. Sci. USA 69: 383-387, 1972. PubMed: 4333982
Tumilowicz JJ, et al. Presence of retrovirus in the B95-8 Epstein-Barr virus-producing cell line from different sources. In Vitro 20: 486-492, 1984. PubMed: 6086497
