| Insert |
DNA: genomic Insert lengths(kb): 5.400000095367432 Tissue: fetal liver Gene product: avian erythroblastosis virus E26 (v-ets) oncogene homolog 1 [ETS1] Alleles: A1, A2, B1, B2, C1, C2, E1, E2, F2, F1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.4, 4.5; AvaI--9.9; HindIII--5.7, 3.4, 0.8; PstI--6.6, 3.3; EcoRI--5.4, 4.5. Contains internal BamHI (0.67 kb) and HindIII (1.2, 1.9, 5.2 kb) sites with distances relative to end closer to ampicillin site. The insert detects a 6.8 kb EcoRI genomic fragment. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. This probe did not detect previously reported SstI RFLPs when the genomic DNA was cut with SacI (an isoschizomer of SstI). This probe was derived from lambda ets H-3. Enzyme(s) not detecting polymorphism: BglI, HaeII, EcoRI, HindIII, MspI, HaeIII, HinfI, HincII, StyI, PstI, PvuII, BglII, BamHI. |
| References |
Aparicio JM, et al. TaqI RFLP linked to the human ets-1 gene. Nucleic Acids Res. 17: 4913, 1989. PubMed: 2568621
de Taisne C, et al. Chromosomal localization of the human proto-oncogene c-ets. Nature 310: 581-583, 1984. PubMed: 6462246
Concannon P, et al. The ataxia-telangiectasia gene (ATA) on chromosome 11 is distinct from the ETS-1 gene. Am. J. Hum. Genet. 46: 789-794, 1990. PubMed: 1969227
Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217
Barbara Weiffenbach, personal communication
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